Description
The AxyPrep MAG Tissue-Blood gDNA Kit utilizes a magnetic beads-based technology to isolate genomic DNA from rodent tail tissues. This protocol provides instruction to extract gDNA from fresh or frozen rodent tails. The protocol is performed in 96-well format and 1.5 mL tubes. Purification begins by the addition of lysis buffer, and Proteinase K to rupture cell membranes and digest protein. Genomic DNA is then immobilized on magnetic particles by the addition of a magnetic binding reagent. This differential binding allows the gDNA to be easily separated from contaminants using a magnetic field. Contaminants can then be rinsed away using a simple washing procedure.
Expanded protocol for animal tissue, cultured cells, buccal swabs, and blood
Features and Specifications:
Restriction enzyme digestion
DNA sequencing
Genotyping
PCR amplification
Product Information:
Qty./Pk: 1 / Pk
Application: Diagnostics
Ordering Information:
MAG-T-GDNA-M, MAG-T-GDNA-S
Packaging:
Kit
Brochure:
Click Here for Brochure
Expanded protocol for animal tissue, cultured cells, buccal swabs, and blood
Features and Specifications:
Restriction enzyme digestion
DNA sequencing
Genotyping
PCR amplification
Product Information:
Qty./Pk: 1 / Pk
Application: Diagnostics
Ordering Information:
MAG-T-GDNA-M, MAG-T-GDNA-S
Packaging:
Kit
Brochure:
Click Here for Brochure
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